Md. Raihan Chowdhury, Kazi Saiful Islam, AGM Rakibuzzaman, Sadia Afrin, Md. Sarowar Jahan
Advanced Chemical Industries Limited, Narayanganj-1400, Bangladesh.
Department of Biochemistry and Molecular Biology, Jahangirnagar University, Savar, Dhaka-1342.
Incepta Pharmaceuticals Ltd, Savar, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka-1000, Bangladesh.
Key words: Direct smears, concentrated smears, Mycobacterium tuberculosis, Pulmonary, PCR IS6110, Sensitivity, Specificity, DOTS (Internationally recommended strategy for TB control; basic component of the Stop TB strategy.
Abstract
Sensitivity and specificity of direct and concentrated AFB smear microscopy has been investigated by PCR analysis and culture methods to determine a rapid and cheap detection of pulmonary tuberculosis. In this study 305 patients were selected and 915 specimens were collected from suspected and hospital admitted patients. Patients were taken from Dhaka Central Jail Hospital and National TB Hospital, Dhaka, Bangladesh. All samples were smeared and Ziehl-Neelsen method and Lowenstein-Jensen (L-J) method were applied. PCR analysis and culture method was used to confirm the detection. Isolated DNA was used in PCR analysis. In this detection study PCR based IS6110 analysis was developed to identify the pulmonary tuberculosis rapidly and cheaply. A total of 915 samples were prepared for analysis and found 70.47% and 82.85% sensitivity in direct and concentrated smears respectively. Both type of smears (direct and concentrated) showed 100% sensitivity and specificity in culture and PCR IS6110 analysis. Since the direct and concentrated smears with the developed method of PCR IS6110 analysis showed significant detection, so that the proposed method was suggested for the detection analysis of Mycobacterium tuberculosis in pulmonary for the developing and poor countries.
Get the original articles in Source: Volume 2, Number 8, August 2012 – IJB
Published By: International Journal of Biosciences (IJB)
