Selection and optimization of bacteriocin production from Escherichia coli and Pseudomonas aeruginosa

Mir Sadiq Shah, Fariha Hasan, Shah Jahan, Rahmat Ali Khan

Department of Biotechnology, University of Science and Technology, Bannu, KPK, Pakistan

Department of Microbiology, Faculty of Biological Sciences, Quaid-I-Azam University, Islamabad, Pakistan

Key words. E. coli, P. aeruginosa, B. cereus, B. subtilis, B. lincolinosis, B. circulans,

probioticbacteria-lactobacillusAbstract

In the present study bacteriocin was produced from Gram negative bacteria (E. coli and P. aeruginosa). These bacterial strains were taken from Microbiology research laboratory Quaid-i-Azam University, Islamabad, and were identified as according to Bergey,s Manual of Determinative Bacteriology (Holt, 1983). Growth was optimized at 25 ºC, 30 ºC, and 37°C for bacteriocin production by shake flask fermentation. Bacteriocin was found to be produced by both P. aeruginosa and E. coli against various related strains of bacillus species used as test organisms. Growth was optimized at different pH (5-9) incubation time (0-144 hours) and glucose concentration of (1-5%) for maximum antibiotics production. Agar well diffusion assay was employed to check antibacterial activity. Maximum antibacterial activity observed at pH 8, 3% glucose concentration and after 96 hours of incubation at 37°C against B. cereus, B. subtilis, B. lincolinosis and B. circulans. Further study will reveal how the intricate relations among antibiotic producing bacteria and their neighbors affect the diversity within microbial communities.

Get the original articles in Source: Volume 2, Number 10 (1), October 2012 – IJB

Published By: International Journal of Biosciences (IJB)

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