Isolation, characterization and identification of roundup degrading bacteria from the soil and gut of Macrotermes michaelseni – IJMM

H.A. Ogot, H. I. Boga, N. Budambula, M. Tsanuo, D.O. Andika, H. J, Ogola

University of Nairobi Box 30197- 00100 Nairobi, Kenya

Taita Taveta University College Box 635-80300 Voi, kenya

Jomo Kenyatta University of Agriculture and Technology Box 62000-00200 Nairobi, Kenya

Pwani University Box 195-80108 Kilifi, Kenya

Jaramogi Oginga Odinga University of Science and Technology, Box 210-40601, Bondo, Kenya

Keywords: Roundup, bioremediation, Macrotermes michaelseni, Enterobacter.

Abstract

Roundup degrading bacteria were isolated from the soil and gut of fungus cultivating termites Macrotermes michaelseni using MM7 media supplemented with the pesticide as the sole source of carbon and energy. The isolates were gram negative rods the isolate from the soil was designated GS1 while that from termite gut was designated GT2. The 16S rRNA gene sequence of the isolates revealed that isolates Both isolates GS1 and GT2 are closely related to Enterobacter sp AY 0822447 with 99% sequence similarity. The results of this study highlight the potential of these bacterial isolates to be used in bioremediation.