The micropropagation of the strawberry cultivar ‘Idea’ – IJAAR

Zoran Nikolic, Katerina Nikolic, Dragan Jankovic, Ana Krstic, Goran Pejkovic

Faculty of Agriculture, University of Priština, Lešak, Serbia

Primary school Radoje Domanovi, Bošnjace, Serbia

Key words: Strawberry, meristem, micropropagation, stolons, multiplication

Abstract

This work presents the production technology of the strawberry cultivar ‘Idea’ by micropropagation. Meristems of 0.5mm size were isolated in June from the stolons of examined strawberry sorts. They were raised on Murashige and Skoog’s (Murashige and Skoog, 1962) medium in presence of 6-Benzylaminopurine (BAP), Indole-3-butyric acid (IBA) and gibberellic acid (GA3). Fifty days later meristems were organized into a foliate rosette. Multiplication was achieved on Murashige and Skoog’s substratum with BAP and IBA. The plants which came to the size of about 10mm were shifted to the substratum for tree rooting. Tree rooting was achieved on Murashige and Skoog’s substratum with IBA. Sixty days later the plants with strongly developed roots were transplanted into the peaty briquettes. One month later the plants were transplanted into flowerpots and kept in a greenhouse at a temperature of 10-150C.

Get the original articles in Source: Volume 4, Number 4, April 2014 – IJAAR

Journal Name: International Journal of Agronomy and Agricultural Research (IJAAR)

Published By: International Network for Natural Sciences

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Effects of different hormones on in vitro regeneration of strawberry (Fragaria x ananassa Duch.)

Tanziman Ara, Rezaul Karim, M. Rezaul Karim, Shahrear Ahmad, Rafiul Islam, Monzur Hossain

Plant Breeding and Gene Engineering Laboratory, Department of Botany, University of Rajshahi, Bangladesh.

Department of Botany, Jagannath University, Bangladesh

Key words: Micropropagation, runner tip, nodal segment and strawberry.

strawberriesAbstract

A simple and rapid protocol for micropropagation of strawberry (Fragaria x ananassa Duch.) was achieved using runner tips and nodal segments. The excised runner tips and nodal segments were cultured on MS medium containing 6-benzylaminopurine, 6-furfuryl amino purine, indole-3-butyric acid and gibberellic acid at various levels of concentration and combination for multiple shoot induction and proliferation. The highest percentage of shoot proliferation was found when both explants (92% from nodal segments and 83% from runner tips) were cultured on MS medium supplemented with 1.5 mg/l 6-benzylaminopurine + 0.5 mg/l 6-furfuryl amino purine. Shoots were rooted most effectively in MS0 medium. Nodal segments were found more responsive explants than runner tips for rapid clonal propagation in strawberry.

Get the original article sin Source: Volume 2, Number 10 (1), October 2012 – IJB

Published By: International Journal of Biosciences (IJB)

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